作为一本相关学科入门的英文教材或重要的参考书，既要考虑基本知识的传授，又要力求反映当前本学科所取得的重要理论成果与进展。《生物工程导论(英文改编版)(第2版)》是在DG Rao《IntroductiOnto BiochemicaIEngineering》基础上，经过重新组织、删减和修改出版的。经过改编，既保留了原作的主要内容和思想，删减了一些与生物化工联系较远的内容，又针对目前国际生物化工领域的热点研究进行了适当补充，以使其内容更加适应符合国内生物化工双语教学需要。本书由DG Rao主编，李春改编。

《生物工程导论(英文改编版)(第2版)》是在DG Rao《IntroductiOnto BiochemicaIEngineering》第二版的基础上，经过编者重新组织、删减和修改出版的。主要供生物类专业低年级学生了解生物工程与技术专业的全貌，明晰后续专业课程之间的相互关系，领悟生物工程领域发展的现状和动态及其对社会和经济发展的影响，尤其是当今社会在面临着能源危机、资源危机和环境危机时生物技术所发挥的作用。阅读本书后，可提高学生对专业知识的理解，激发其进一步学习专业知识的兴趣和爱好。对于其他相关专业的学生则可拓展其视野、优化其知识结构、提高其科学素养。

《生物工程导论(英文改编版)(第2版)》既可作为高等院校生物工程、生物技术、化学工程、制药工程和环境工程等专业的导论教材，化学、生物和食品等专业的拓展教材，也可供相关学科从事教学、科研和生物产业管理者学习和参考。本书由DG Rao主编，李春改编。

Chapter 1 Introduction to Bioprocessing Fundamentals 1

1.1 HISTORICAL DEVELOPMENTS OF BIOPROCESSING TECHNOLOGY 1

1.2 OVERVIEW OF TRADITIONAL AND MODEN APPLICATIONS OF BIOTECHNOLOGY 3

1.3 INTERADISCIPLINARY APPROACH TO BIOPROGROCESSING 3

1.4 OUTLINES OF LINTEGRATED BIOPROCESS 4

1.5 UNIT OPERATIONS BIOPROCESS 6

References 7

Review Questions 7

Chapter 2 Overview of Microbiology 8

2.1 HISTORIC BACKGROUND 8

2.2 MICROSCOPY 10

2.3 MICROBIAL TAXONOMY 10

2.4 CHEMICAL COMPOSITION 13

2.5 NUTRITIONAL REQUIREMENTS 13

2.6 METABOLISM 15

2.7 PROCARYOTIC CELL 19

2.8 EUCARYOTIC CELL 22

2.9 VIRUSES 26

2.10 FUNGI 26

2.11 ALGAE 26

2.12 PROTOZOA 27

2.13 IMPORTANCE OF MICROBIOLOGY 27

2.14 CONCLUDING REMARKS 29

References 29

Review Questions 29

Chapter 3 Introduction to Biochemistry 31

3.1 LIPIDS 31

3.2 PROTEINS 36

3.3 CARBOHYDRATES 39

3.4 NUCLEIC ACIDS 43

3.5 VITAMINS 45

References 50

Review Questions 50

Chapter 4 Enzymes 51

4.1 HISTORY OF ENZYMES 51

4.2 CLASSIFICATION OF ENZYMES 51

4.3 ENZYMES AS BIOLOGICAL CATALYSTS 52

4.4 ENZYME SPECIFICITY 54

4.5 ENZYME KINETICS 56

4.6 IMMOBILIZATION OF ENZYMES 60

4.7 INDUSTRIAL APPLICATIONS OF ENZYMES 63

References 67

Review Questions 68

Chapter 5 Fermentation 69

5.1 GENERAL REQUIREMENTS OF FERMENTATION PROCESS 69

5.2 RANGE OF FERMENTATION PROCESS 70

5.3 DESIGN AND CONSTRUCTION OF FERMENTER 76

5.4 MEDIA DESIGN FOR FERMENTATION 78

5.5 STERILIZATION 84

5.6 AEROBIC AND ANAEROBIC FERMENTATION PROCESSES 90

5.7 SOLID STATE AND SUBMERGED FERMENTATION AND THEIR APPLICATIONS 91

5.8 VARIOUS TYPES OF BIOREACTORS 92

References 98

Review Questions 100

Chapter 6 Kinetics of Microbial Growth and Biochemical Reactors 101

6.1 PHASES OF CELL GROWTH 102

6.2 BATCH REACTOR DATA ANALYSIS 102

6.3 KINETIC MODELS FOR CELL GROWTH 107

6.4 GROWTH OF FILAMENTOUS ORGANISMS 112

6.5 SUBSTRATE AND PRODUCT INHIBITION ON CELL GROWTH 113

6.6 STRUCTURED MODELS 114

6.7 DESIGN EQUATIONS BASED ON BIOCHEMICAL REACTIONS 116

References 119

Review Questions 120

Problems 120

Chapter 7 Ideal Reactors 121

7.1 DESIGN OF IDEAL REACTORS 121

7.2 SINGLE REACTOR 124

7.3 MULTIPLE REACTORS 131

References 136

Review Questions 137

Problems 137

Chapter 8 Multiple Reactions 138

8.1 PARALLEL REACTIONS 139

8.2 SERIES REACTIONS 141

8.3 SERIES PARALLEL REACTIONS 145

8.4 DESIGN PRINCIPLES 147

References 149

Review Questions 149

Chapter 9 Heat Transfer in Bioprocessing 151

9.1 HEAT TRANSFER BY CONDUCTION 152

9.2 HEAT TRANSFER BY CONVECTION 159

9.3 HEAT TRANSFER BY RADIATION 171

References 176

Review Questions 177

Problems 177

Chapter 10 Mass Transfer in Bioprocessing Operations 179

10.1 MASS TRANSFER BY DIFFUSION 180

10.2 THEORIES OF DIFFUSIONAL MASS TRANSFER 180

10.3 MASS TRANSFER BY CONVECTION 183

10.4 OXYGEN TRANSFER METHODOLOGY IN FERMENTERS 191

10.5 FACTORS AFFECTING OXYGEN TRANSFER RATE 195

References 202

Review Questions 203

Problems 203

Chapter 11 Heterogeneous Reaction Systems 205

11.1 MASS TRANSFER CONSIDERATIONS 205

11.2 INTRA PARTICLE DIFFUSION AND REACTION RATE 208

11.3 EFFECTIVENESS FACTOR AND THIELE MODULUs 210

11.4 OBSERVABLE THIELE MODULUS 213

11.5 BIOREACTOR SELECTION CRITERIA 214

References 215

Review Questions 215

Chapter12 Bioreactors and Fermentation 217

12.1 BIOREACTORS 217

12.2 MONITORING AND CONTROL OF FERMENTATION PROCESSES 219

12.3 VARIOUS ACCESSORIES 224

12.4 CULTIVATION OF ORGANISMS 225

12.5 MEDIA OPTIMISATION 229

References 230

Review Questions 231

Chapter 13 Product Recovery 232

13.1 REMOVAL OF SUSPENDED SOLIDS 234

13.2 FILTRATION 234

13.3 SEDIMENTATION 241

13.4 CENTRIFUGATION 244

13.5 CELL DISRUPTION 248

13.6 EXTRACTION 249

13.7 MEMBRANE SEPARATION 252

13.8 CHROMATOGRAPHY 253

13.9 CRYSTALLISATION 256

13.10 DRYING 258

References 265

Review Questions 265

Chapter 14 Effluent Treatment 267

14.1 NEED FOR EFFLUENT TREATMENT 267

14.2 PHYSICAL METHODS 269

14.3 CHEMICAL METHODS 269

14.4 BIOLOGICAL METHODS 270

References 275

Review Questions 275

Chapter 15 Design and Analysis of Bioreactors 276

15.1 STABILITY AND ANALYSIS OF BIOREACTORS 277

15.2 DESIGN AND OPERATION OF BIOREACTORS 279

15.3 BIOREACTOR FOR PLANT AND ANIMAL CELLS 286

15.4 SCALE UP OF BIOREACTORS 289

15.5 SOME CRITERIA FOR SELECTION OF BIOREACTORS 293

References 295

Review Questions 296

Chapter 16 Bioprocess Economics 298

16.1 PLANTDESIGN AND ECONOMICS 300

16.2 COST OF PRODUCTION 303

16.3 BREAK EVEN ANALYSIS 305

16.4 PROJECT ECONOMICS 307

16.5 DEPRECIATION 310

16.6 PROJECT ECONOMICS FOR CITRIC ACID MANUFACTURE 311

References 319

Review Questions 319