Preface

Chapter 1 Introduction

 1.1 Conception of Enzyme and Its Development

 1.2 The Characteristics of Enzyme Action

 1.3 Factors that Affecting Enzyme Action

 1.4 Classification and Nomenclature of Enzymes

 1.5 Determination of Enzyme Activity

 1.6 Methods for Enzymes Production

 1.7 Development Survey of Enzyme Engineering

 Review and Thinking

Chapter 2 Enzyme Production by Microbial Fermentation

 2.1 Regulation of Enzyme Biosynthesis in Microbial Cells

 2.2 Characteristic of Microbes for Enzyme Production

 2.3 Fermentation Process Conditions and Its Control

 2.4 Enzyme Fermentation Kinetics

 2.5 Enzyme Production by Immobilized Cell Fermentation

 2.6 Enzyme Production by Immobilized Protoplast Fermentation

 Review and Thinking

Chapter 3 Enzyme Production by Animal and Plant Cell Cultures

 3.1 The Regulation of Enzyme Biosynthesis in Animal and Plant Cells

 3.2 Enzyme Production by Plant Cell Culture

 3.3 Enzyme Production by Animal Cell Culture

 Review and Thinking

Chapter 4 Extraction, Separation and Purification of Enzymes

 4.1 Cell Disruption

 4.2 Enzyme Extraction

 4.3 Precipitation Separation

 4.4 Centrifugal Separation

 4.5 Filtration and Membrane Separation

 4.6 Chromatographic Separation

 4.7 Eleetrophoresis Separation

 4.8 Extraction Separation

 4.9 Crystallization

 4.10 Concentration and Drying

 Review and Thinking

Chapter 5 Enzyme Molecular Modification

 5.1 Metal Ion Substitution Modification

 5.2 Macromolecular Binding Modification

 5.3 Side Chain Group Modification

 5.4 Peptide Chain Limited Hydrolysis Modification

 5.5 Nucleotide Chain Cleavage Modification

 5.6 Amino Acid Substitution Modification

 5.7 Nucleotide Substitution Modification

 5.8 Physical Modification

 5.9 Application of Enzyme Molecular Modification

 Review and Thinking

Chapter 6 Immobilization of Enzymes, Cells and Protoplasts

 6.1 Enzyme Immobilization

 6.2 Cell Immobilization

 6.3 Protoplast Immobilization

 Review and Thinking

Chapter 7 Enzyme Catalysis in Non-aqueous Phases

 7.1 The Main Scopes of Enzyme Catalysis in Non-aqueous Phase

 7.2 Effects of Water and Organic Solvents on Enzyme Catalysis in Organic Media

 7.3 The Catalytic Characters of Enzymes in Organic Media

 7.4 Conditions of Enzyme Catalytic Reaction in Organic Media and Its Control

 7.5 Application of Enzyme Catalysis in Non-aqueous Phase

 Review and Thinking

Chapter 8 Enzyme Directed Evolution

 8.1 The characteristics of Enzyme Directed Evolution

 8.2 Random Mutation of Enzyme Gene

 8.3 Directed Selection of Enzyme Gene Mutants

 8.4 The Applications of Enzyme Directed Evolution

 Review and Thinking

Chapter 9 Enzyme Reactors

 9.1 Types of Enzyme Reactors

 9.2 Choice of Enzyme Reactors

 9.3 Design of Enzyme Reactors

 9.4 Enzyme Reactor Operation

 Review and Thinking

Chapter 10 Applications of Enzymes

 10.1 Enzymes Application in Pharmaceutical Industry

 10.2 Application of Enzymes in Food

 10.3 Enzyme Application in Light and Chemical Industries

 10.4 Enzyme Application in Environmental Protection

 10.5 Enzyme Application in Biotechnology

 Review and Thinking

Main references

《酶工程(第3版)》简介：Enzyme Engineering (Third Edition) introduces the basic theories, basic technologies recent developments and growth trends in the fields of enzyme production and applications. This book in 10 chapters covers the introduction, enzyme production by microbial fermentation, enzyme production by animal and plant cell cultures, extraction, separation and purification of enzymes, enzyme molecular modification, immobilization of enzymes, cells and protoplasts, enzyme catalysis in non-aqueous phases, enzyme directed evolution, enzyme reactors, and applications of enzymes. A review and thinking issues are given at the end of every chapter.

This book can be used as a text book for the students specializing in biotechnology, bioengineering, biochemical engineering, enzyme engineering, fermentation engineering, bioscience etc. It can also be used as reference by the teachers, scientists, engineers and technicians working in the fields related to enzyme engineering. This book has been translated from the third Chinese edition of "Enzyme Engineering" published by Science Press, Beijing, China.

《酶工程(第3版)》由Guo Yong所著。

This book was translated from Chinese "Enzyme Engineering" (Third Edition) published in 2009 by Science Press, Beijing, China. The contents of the book include 10 chapters which are; chapter 1: introduction; chapter 2. Enzyme production by microbial fermentation; chapter 3. Enzyme production by animal and plant cell culture; chapter 4. Extraction, isolation and purification of enzymes; chapter 5. Enzyme molecular modifications; chapter 6. Immobilization of Enzymes, Cells and Protoplasts; chapter 7. Enzyme catalysis in non-aqueous phases; chapter 8. Enzyme directed evolutions; chapter 9. Enzyme reactors and chapter 10. Applications of enzymes.

《酶工程(第3版)》由Guo Yong所著。