本书介绍了生物修复的原理和应用土壤污染物的策略和毒性评估。这本书特别关注重金属和持久性有机污染物（持久性有机污染物），如多环芳烃和杀虫剂。这些技术包括植物修复、纯培养、DNA稳定同位素探测（DNA-SIP）、磁性纳米粒子介导的分离（MMI）和全细胞生物搬运工。
本书可作为科研人员、管理人员和研究生、生态和生物技术人员参考。

1 Effective phytoremediation of low-level heavy metals by native macrophytes in a vanadium mining area, China
1.1 Overview
1.2 Site description
1.3 Heavy metal distribution in the vanadium contaminated area
1.4 Uptake of heavy metals in macrophytes in the vanadium mining area
1.5 Heavy metal uptake by native aquatic plants in laboratory hydroponics
1.6 Conclusion
2 Microbial degradation of organophosphorus pesticides via pure cultivation approach: novel degraders, kinetics and functional genes
2.1 Overview
2.2 Isolation and characterization of OPs-degrading microorganisms
2.3 Effects of pH and temperature on OPs biodegradation
2.4 Kinetic analysis of OPs degradation
2.5 Identification of OPs-degrading genes
2.6 Genotoxicity assessment of OPs and their degradation products by the whole-cell bioreporter ADP1_recA
2.7 Conclusion
3 Cultivation-independent approaches for the identification of functional microorganisms-taking PAHs as examples
3.1 Overview
3.2 PAHs-degrading microorganisms and genes
3.3 Fluorescence in situ hybridization (FISH)
3.4 Stable isotope probing (SIP)
3.5 Metagenomics based on high throughput sequencing
3.6 Transcriptomics
3.7 Single-cell technology
3.8 Magnetic-nanoparticle mediated isolation (MMI)
4 Unraveling uncultivable pesticide degraders via stable isotope probing (SIP)
4.1 Overview
4.1.1 Application and impacts of pesticides
4.1.2 Microbial degradation of pesticides
4.2 Stable isotope probing for identifying functional microbes
4.2.1 Principles of stable isotope probing
4.2.2 SIP as a promising tool in identifying functional microbes
4.3 Application of SIP in identifying active pesticide degraders and studying bacterial diversity and function
4.3.1 Direct application with stable isotope labeled pesticide
4.3.2 Application with stable isotope in pesticide metabolites and similar compounds
4.4 Technical details of applying DNA-SIP in identifying functional pesticide degraders
4.4.1 In situ inoculation
4.4.2 Extraction and fractionation
4.4.3 Sequencing and analysis
4.5 Combination of DNA-SIP with other techniques
4.5.1 Coupling with sequencing-based approaches
4.5.2 Coupling with single cell techniques
4.5.3 Coupling with other approaches for unraveling microbial functions
4.6 Conclusion
5 Identification of chlorpyrifos-degrading microorganisms in farmland soils via cultivation-independent and
cultivation-dependent approaches
5.1 Overview
5.2 CPF biodegradation in soil
5.3 MNPs synthesis and functionalization of soils
5.4 Microorganisms responsible for CPF biodegradation identified by MMI
5.5 Isolation of CPF-degraders by pure cultivation
5.6 Identification of organophosphorus hydrolase (OPH) genes affected in CPF metabolism
5.7 The degradation metabolite of CPF
5.8 Comparison between the degraders isolated via MMI and pure cultivation
5.9 Conclusion
6 Bioaugmentation performance of cultivable and uncultivable microorganisms for pyrene degradation
6.1 Overview
6.2 Biodegradation and bioaugmentation performance
6.3 Microbial community diversity and structure during pyrene degradation process
6.4 Pyrene degraders revealed by cultivation-dependent and cultivation-independent methods
6.5 Intra-correlation within soil bacterial community
6.6 Dynamics of pyrene dioxygenase encoding genes
6.7 Metabolites and degradation pathway of pyrene
6.8 Comparative analysis of the bioaugmentation performance of PAHs degraders isolated via MMI and pure cultivation
6.9 Conclusion
7 A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples
7.1 Overview
7.2 Preparation, sampling and chemical analysis of soil and seawater samples
7.3 SOS model simulation
7.4 Modelling of the E.coli bioreporter response to art