《蜜蜂生物学理论中若干问题研究》是江西农业大学蜜蜂研究所曾志将教授研究团队取得的研究成果。曾志将教授是中国养蜂学会副理事长、著名蜜蜂生物学专家、国家蜂产业技术体系岗位科学家。多年来，在国家蜂产业技术体系、国家自然科学基金等项目资助下，曾志将教授带领团队成员，围绕蜜蜂生物学这一主题进行了系统研究，并在BMC genomics、PLoS One、Naturwissenschafien、Apidologie等世界高水平期刊发表诸多学术论文，取得了许多原创性研究成果。

本书系统介绍了蜜蜂生物学中的蜜蜂级型分化与差异表达、工蜂劳动分工、蜜蜂发育生物学、蜜蜂性等位基因多态性分析、蜜蜂学习与记忆、工蜂监督、辨认与优惠六个领域研究进展。

曾志将等编著的《蜜蜂生物学理论中若干问题研究》是江西农业大学蜜蜂研究所曾志将教授团队在国家蜂产业技术体系、国家自然科学基金等项目连续资助下取得的研究成果。内容包括蜜蜂级型分化与基因差异表达，工蜂劳动分工，蜜蜂遗传与发育，蜜蜂性等位基因多态性分析，蜜蜂学习与记忆，工蜂监督、辨认与优惠等蜜蜂生物学主题。

本书知识新颖，理论性强，可供广大生物科技工作者及相关专业研究生参考使用，读者可藉此了解到很多蜜蜂生物学新知识。

序

前言

Ⅰ.蜜蜂级型分化与基因差异表达

 1.Diet and cell size both affect queen—worker differentiation through DNA

 methylation in honey bees

 2.Genome—wide analysis indicates that queen larvae have lower methylation levels in the honey beel

 3.Differentially expressed microRNAs between queen and worker larvae of honey beel

 4.Expression of sir2，hdac l and ash2 in honey bee（Apis mellifera L.）queens and Workers

 5.中华蜜蜂DNA甲基化转移酶Dnmt3基因克隆及表达谱分析

 6.中华蜜蜂dynactin p62基因的克隆及不同发育阶段表达分析

Ⅱ.工蜂劳动分工

 1.A comparison of digital gene expression profiling and methyl DNA immunoprecipitation as methods for gene discovery in honeybee（Apis mellifera）behavioural genomic analyses

 2.Behaviour and molecular physiology of nurses of worker and queen larvae in honey bees（Apis mellifera）

 3.Assessment of flight activity and homing ability in Asian and European honey bee species，Apls cerana and Apis melliJ'era，measured with radio frequency tags

 4.基于RFID技术的西方蜜蜂采集行为研究

Ⅲ.蜜蜂遗传与发育

 1.Transcriptome analysis ofthe Asian honey bee Apis cerana

 2.Transcriptome difference in hypopharyngeal gland between Western Honeybees（Apis mellifera）and Eastern Honeybees

 3.A SNP based highdensity linkage map of AptS cerana reveals a high recombination rate similar to Apis mellifera

 4.Epigenetic modification of gene expression in honey bees by heterospecific gland secretions

 5.Changes in alternative splicing in Apis mellifera bees fed Apis cerana royal jelly

 6.Nutrition affects longevity and gene expression in honey bee（Apis mellifera）workers

 7.Effects of 10一Hydroxy2decenoic acid on the development of honeybee（Apis mellifera）larvae

 8.Mating flight causes genome—wide transcriptional changes in sexually mature honeybee queens

 9.蜂王浆对雌性蜜蜂幼虫dynatin p62基因甲基化影响

 10.婚飞行为影响中华蜜蜂性成熟处女蜂王的基因表达I

 11.婚飞对中华蜜蜂性成熟处女蜂王sRNAs表达的影响

Ⅳ.蜜蜂性等位基因多态性分析

 1.The sex determination gene shows no founder effect in the giant honey bee，Apis dorsata

 2.Polymorphism analysis ofcsd gene in six Apis mellifera subspecies

 3.csd alleles in the red dwarfhoney bee（Apisflorea，Apidae，Hymenoptera）show exceptionally high nucleotide diversity

 4.Nucleotide diversity based on csd gene of the black giant honey bee（Hymenoptera：Apidae：A.1 aboriosa）

 5.中华蜜蜂csd基因的多态性分析

Ⅴ.蜜蜂学习与记忆

 1.Comparison of learning and memory ofApis cerana and Apis mellifera

 2.Cross—modal learning between visual and olfactory in Apis cerana

 3.Gene expression analysis following olfactory learning in Apis mellifera

 4.The integrative analysis of microRNA and mRNA expression in Apis mellifera following mazebased visual pattern learning

Ⅵ.工蜂监督、辨认与优惠（Worker Policing，Discrimination and Nepotism）

 1.Does the queen mark pheromone in the wall of cell？

 2.Decision making plasticity of swarming honeybees

 3.应用微卫星DNA技术研究中华蜜蜂群内工蜂监督效果

 4.中华蜜蜂卵表面微观结构及化学成分初步研究

 5.中华蜜蜂急造王台的工蜂亲属优惠研究

 6.中华蜜蜂幼虫信息素鉴定

 7.幼虫信息素中三种酯类对中华蜜蜂工蜂发育和采集行为的影响

 8.幼虫信息素中三种酯类对中华蜜蜂和意大利蜜蜂工蜂哺育和封盖行为及蜂王发育影响