本书详细论述了光学光谱理论，并介绍了如何将这些理论运用于现代分子和细胞生物物理和生物化学。全书共分12章，内容包括：电子和振动吸收，荧光共振能量转移，激广相互作用，圆二色性，相干和失相，超快泵探针和光子回波光谱，单分子和荧光相关光谱，拉曼散射，以及多重吸收。

本书可供分析化学、生物化学、生物物理学和物理化学等专业高校师生、科研人员参考。

本书详细论述了光学光谱理论，并介绍如何将这些理论运用于现代分子和细胞生物物理和生物化学。内容包括电子和振动吸收，荧光共振能量转移，激广相互作用，圆二色性，相干和失相，超快泵探针和光子回波光谱，单分子和荧光相关光谱，拉曼散射，以及多重吸收。

本书论述基于时间相关的量子力学，为此领域专业人士提供了足够全面和详细的资料，并例举了数量众多的理论表达式或演示实验等。

本书可供分析化学、生物化学、生物物理学和物理化学等专业高校师生、科研人员参考。

1 Introduction

  1.1 Overview

  1.2 The Beer-Lambert Law

  1.3 Regions of the Electromagnetic Spectrum

  1.4 Absorption Spectra of Proteins and Nucleic Acids

  1.5 Absorption Spectra of Mixtures

  1.6 The Photoelectric Effect

  1.7 Techniques for Measuring Absorbance

  1.8 Pump-Probe and Photon-Echo Experiments

  1.9 Linear and Circular Dichroism

  1.10 Distortions of Absorption Spectra by Light Scattering or Nonuniform Distributions of the Absorbing Molecules

  1.11 Fluorescence

  1.12 IR and Raman Spectroscopy

  1.13 Lasers

  1.14 Nomenclature

2 Basic Concepts of Quantum Mechanics

  2.1 Wavefunctions, Operators, and Expectation Values

 2.1.1 Wavefunctions

 2.1.2 Operators and Expectation Values

  2.2 The Time-Dependent

 and Time-Independent Schr6dinger Equations

 2.2.1 SuperpositionStates

  2.3 Spatial Wavefunctions

 2.3.1 A Free Particle

 2.3.2 A Particle in a Box

 2.3.3 The Harmonic Oscillator

 2.3.4 Atomic Orbitals

 2.3.5 Molecular Orbitals

 2.3.6 Approximate Wavefunctions for Large Systems

  2.4 Spin Wavefunctions and Singlet and Triplet States

  2.5 Transitions Between States: Time-Dependent Perturbation Theory

  2.6 Lifetimes of States and the Uncertainty Principle Light

  3.1 Electromagnetic Fields

 3.1.1 Electrostatic Forces and Fields

 3.1.2 Electrostatic Potentials

 3.1.3 Electromagnetic Radiation

 3.1.4 Energy Density and Irradiance

 3.1.5 The Complex Electric Susceptibility and Refractive Index

 3.1.6 Local-Field Correction Factors

  3.2 The Black-Body Radiation Law

  3.3 Linear and Circular Polarization

  3.4 Quantum Theory of Electromagnetic Radiation

  3.5 Superposition States and Interference Effects in Quantum Optics

  3.6 Distribution of Frequencies in Short Pulses of Light Electronic Absorption

  4.1 Interactions of Electrons with Oscillating Electric Fields

  4.2 The Rates of Absorption and Stimulated Emission

  4.3 Transition Dipoles and Dipole Strengths

  4.4 Calculating Transition Dipoles for n Molecular Orbitals

  4.5 Molecular Symmetry and Forbidden and Allowed Transitions

  4.6 Linear Dichroism

  4.7 Configuration Interactions

  4.8 Calculating Electric Transition Dipoles with the Gradient Operator

  4.9 Transition Dipoles for Excitations to Singlet and Triplet States

  4.10 The Born-Oppenheimer Approximation, Franck-Condon Factors, and the Shapes of Electronic Absorption Bands

  4.11 Spectroscopic Hole-Burning

  4.12 Effects of the Surroundings on Molecular Transition Energies

  4.13 The Electronic Stark Effect

5 Fluorescence

  5.1 The Einstein Coefficients

  5.2 The Stokes Shift

  5.3 The Mirror-Image Law

  5.4 The Strickler-Berg Equation and Other Relationships Between Absorption and Fluorescence

  5.5 Quantum Theory of Absorption and Emission

  5.6 Fluorescence Yields and Lifetimes

  5.7 Fluorescent Probes and Tags 

  5.8 Photobleaching 

  5.9 Fluorescence Anisotropy

  5.10 Single-Molecule Fluorescence and High-Resolution Fluorescence Microscopy

  5.11 Pluorescence Correlation Spectroscopy

  5.12 Intersystem Crossing, Phosphorescence, and Delayed Fluorescence

6 Vibrational Absorption

  6.1 Vibrational Normal Modes and Wavefunctions

  6.2 Vibrational Excitation

  6.3 IR Spectroscopy of Proteins

  6.4 Vibrational Stark Effects Resonance Energy Transfer

  7.1 Introduction

  7.2 The F6rster Theory

  7.3 Exchange Coupling

  7.4 Energy Transfer to and from Carotenoids in Photosynthesis Exciton Interactions

  8.1 Stationary States of Systems with Interacting Molecules

  8.2 Effects of Exciton Interactions on the Absorption Spectra of Oligomers

  8.3 Transition-Monopole Treatments of Interaction Matrix Elements and Mixing with Charge-Transfer Transitions

  8.4 Exciton Absorption Band Shapes and Dynamic Localization of Excitations

  8.5 Exciton States in Photosynthetic Antenna Complexes

  8.6 Excimers and Exciplexes Circular Dichroism

  9.1 Magnetic Transition Dipoles and n-n* Transitions

  9.2 The Origin of Circular Dichroism

  9.3 Circular Dichroism of Dimers and Higher Oligomers

  9.4 Circular Dichroism of Proteins and Nucleic Acids

  9.5 Magnetic Circular Dichroism 

10 Coherence and Dephasing

  10.1 Oscillations Between Quantum States of an Isolated System

  10.2 The Density Matrix

  10.3 The Stochastic Liouville Equation

  10.4 Effects of Stochastic Relaxations on the Dynamics of Quantum Transitions

  10.5 A Density-Matrix Treatment of Steady-State Absorption

  10.6 The Relaxation Matrix

  10.7 More General Relaxation Functions and Spectral Lineshapes

  10.8 Anomalous Fluorescence Anisotropy

11 Pump-Probe Spectroscopy, Photon Echoes,and Vibrational Wavepackets

  11.1 First-Order Optical Polarization

  11.2 Third-Order Optical Polarization and Nonlinear Response Functions

  11.3 Pump-Probe Spectroscopy

  11.4 Photon Echoes

  11.5 Transient Gratings

  11.6 Vibrational Wavepackets

  11.7 Wavepacket Pictures of Spectroscopic Transitions

12 Raman Scattering and Other Multiphoton Processes

  12.1 Types of Light Scattering

  12.2 The Kramers-Heisenberg-Dirac Theory

  12.3 The Wavepacket Picture of Resonance Raman Scattering

  12.4 Selection Rules for Raman Scattering

  12.5 Surface-Enhanced Raman Scattering

  12.6 Biophysical Applications of Raman Spectroscopy

  12.7 Coherent Raman Scattering

  12.8 Multiphoton Absorption

  12.9 Quasielastic (Dynamic) Light Scattering (Photon Correlation Spectroscopy)

Appendix 1 - Vectors

Appendix 2 - Matrices

Appendix 3 - Fourier Transforms

Appendix 4 - Fluorescence Phase Shift and Modulation

Appendix 5 - CGS and SI Units and Abbreviations

References

Subject Index