Plant Virology, the study of plant viruses and their diseases, is an important branch of science comprised of challenges and opportunities in China and developing countries, where agriculture is of key importance to date. The discovery of principles and the development of new techniques are helpful in feeding the population more safely and in providing more healthy systems of food production,along with many other aspects of social life such as ornamentals and natural medicine.

Experimental Plant Vitology provides the updated methodology for studying the genomic characterization and mechanisms of infection,the quantitative determination as well as the diagnosis of plant pathogenic viruses. With illustrations showing viral symptoms and ultra-structures, clear and concise descriptions, the book presents thelatest developments in experimental plant virology.

This book is intended for researchers, university teaching staff,graduate students and undergraduates in plant science.

1 Gene Cloning of Cucumber Mosaic Virus and Some Related Viral Agents

 1.1 Introduction

 1.2 A Tomato Strain of Cucumber Mosaic Virus, a Natural Reassortant Between Subgroups IA and II

 1.3 The Araceae Strain of Cucumber Mosaic Virus Infecting Pinellia ternate Suggested to be a Novel Class Unit Under Subgroup I

1.3.1 Phylogenetice and Sequence Divergence Analysis of 3a and CP ORFs

1.3.2 Phylogenetice and Sequence Divergence Analysis of 5' UTR and 3' UTR, 2a and 2b ORFs of RNA3

 1.4 The Potyvirus Infecting PinelIia ternata is a Recombinant Contributed by Soybean Mosaic Virus and Lettuce Mosaic Virus

1.4.1 DAS-ELISA Analysis of Field Samples for Detecting the Potyvirus

1.4.2 Sequencing and Nucleotide Sequence Analysis of the Potyvirus Infecting Pinellia

1.4.3 Amino Acid Sequence Analysis for CP of the Potyvirus Infecting Pinellia

1.4.4 Nucleotide Sequence Analysis for CP N-terminal of the Potyvirus Infecting Pinellia

1.4.5 Amino Acid Sequences for N-terminal and for the Conserved Region of the Potyvirus Infecting Pinellia

1.4.6 Nucleotide Sequences for 3' UTR of the Potyvirus Infecting Pinellia

1.4.7 The General Character and Possible Origin of the Potyvirus Infecting Pinellia

 1.5 The 5' Terminal and a Single Nucleotide Determine the Accumulation of Cucumber Mosaic Virus Satellite RNA

1.5.1 GUUU- in 5' Terminal is Necessary to Initiate Replication of 2msatRNA

1.5.2 Typical Structure at the 5' Terminal is Necessary for Long-distance Movement or High Accumulation of 2msatRNA

1.5.3 Low Accumulation of 2mF5sat Mutants is Related to Single Nucleotide Mutation

1.5.4 Secondary Structure of 2mF5sat Impaired its Replication Capacity

 1.6 Methodology

1.6.1 Purification of CMV Virions from Plant Tissue

1.6.2 RT-PCR and cDNA Cloning for Full-length Genomic RNAs of Cucumber Mosaic Virus

1.6.3 RT-PCR and Gene Cloning for 3'-end of Viral Genome of Soybean Mosaic Virus

1.6.4 Sequence Analysis and Phylogenetice Analysis

1.6.5 Pseudo-recombination of Satellite RNA of Cucumber Mosaic Virus and the Helper Virus

 References

2 Molecular Detection of Cucumber Mosaic Virus and Other RNA Viruses Based on New Techniques

3 Infectious Clones and Chimerical Recombination of Cucumber Mosaic Virus and its Satellite RNAs

4 Gene Function of Cucumber Mosaic Virus and its Satellite RNA Regarding Viral-host iNteractions

5 Plant MicroRNAs and Response of Infection of Plant Viruses

6 Genomic Characterization of Zew Viruses with Double Stranded RNA Genomes

Index