王武主编的《生物技术概论（双色版）》为国家双语教学示范课程配套教材，全书分为基因克隆、细胞工程、酶技术、工业发酵四个部分，共21章，分别介绍了上述四大生物技术领域的基础知识、技术关键以及发展前沿与动态，并对有关技术的正负面影响提出思辨。附录部分提供了关键词汇的中英文对照；列出了本领域诺贝尔奖获得者名单及其主要贡献；提供了近十年来出版的英文原版专业书目，以利于读者自学、参考。

王武主编的《生物技术概论（双色版）》为国家双语教学示范课程配套教材，全书分为基因克隆、细胞工程、酶技术、工业发酵四个部分，共21章，分别介绍了上述四大生物技术领域的基础知识、技术关键以及发展前沿与动态，并对有关技术的正负面影响提出思辨。附录部分提供了关键词汇的中英文对照；列出了本领域诺贝尔奖获得者名单及其主要贡献；提供了近十年来出版的英文原版专业书目，以利于读者自学、参考。

《生物技术概论（双色版）》主要供相关专业本科生双语课程使用，也适合作为研究生或高职高专优秀生的选用教材。对于生物技术产业的技术人员，以及准备出国深造的青年学子而言，本书也具有一定的参考价值。

Preface

Part I Gene Cloning

Chapter 1 Introduction to Gene Cloning

1.1 Brief Overview

1.2 Historical Events

1.3 Significance and Alertness

Chapter 2 Gene and Genome

2.1 Discovery of Hereditary Factor

2.2 Basic Knowledge of Gene

2.3 Sequence Signals Involved with Gene Expression

2.4 About Genomic and Metagenomic Studies

Chapter 3 Basic Techniques for Experiments with DNA

3 1 Enzymatic Tools for Gene Manipulation

3.20ligonucleotide Tools

3.3 DNA Purification

3.4 Electrophoresis

3.5 In Vitro Synthesis of DNA

3.6 DNA Sequencing

3.7 Macromolecular Hybridization and Blotting

3.8 PCR Techniques

Chapter 4 Cloning Procedure

4.1 Basic Procedure of Cloning

4 2 Selection of Cloning Vectors

4.3 Preparation of Donor DNA

4.4 Joining of Donor and Vector by Ligation

4.5 Selection Host Cell

4.6 Introduction of Recombinant DNA into Host Cells

4.7 "Selection, Screening and Subcloning

4.8 Derivative Technology Based on Cloning

Chapter 5 Application and Impacts

5.1 Initiation of Recombinant DNA Industry

5.2 General Aspects of Application

5.3 Contribution and Impact

Part II Cell Engineering

Chapter 6 Introduction on Cell Engineering

6.1 Brief Overview

6.2 Essentials of Cell Engineering

6.3 Progress Clues

Chapter 7 Cell Fusion and Hybridization

7.1 General Aspects

7.2 Protoplast Technique

7.3 Basic Methods of Cell Fusion

7.4 Fusant Screening

7.5 Application of Cell Fusion

Chapter 8 Plant Tissue and Cell Culture

8.1 Plant Tissue Culture System

8.2 Gene Transfer to Plants

8.3 Artificial Seeds and Virusfree Plants

Chapter 9 Animal Tissue and Cell Culture

9.1 Establishment of Animal Tissue Culture System

9.2 Gene Transfer to Animal Cell

9.3 Animal Hybrids and Hybridoma

9.4 Approaches of Stem Cell Research

Chapter 10 Application Examples from Cell Engineering

10.1 Bioproducts from Cell Engineering

10.2 Breeding of New Agronomic Varieties

10.3 Transgenic Animals as Models for Medical Research

Part III Enzyme Technology

Chapter 11 Fundamental Nature of Enzyme

11.1 Definition, Basic Nature and Classification of Enzyme

11.2 Determination of Enzyme Activities

11.3 Factors Affecting Enzyme Activity

11.4 Resources of Enzyme

11.5 Uses of Enzyme

Chapter 12 Enzyme Biosynthesis and Products

12.1 BiDresources of Enzyme

12.2 About Enzyme Synthesis

12.3 Enzyme Fermentation and Products

Chapter 13 Enzyme Recovery& Purification

13.1 Pretreatment of Crude Enzyme Sample

13.2 Removing of Impurities and, Sample Concentration

13.3 Chromatographic Separation

13.4 Polishing of Enzyme

13.5 Rational Formulation of Final Enzyme Products

Chapter 14 Enzymatic Conversion

14.1 Enzymatic Conversion

14.2 Immobilization of Enzyme and Cells

14.3 Enzymatic conversion in nonaqueous Phase

Chapter 15 New Approaches of Enzyme Technology

15.1 Modification on Enzymes

15.2 Enzyme Carriers for Immobilization

15.3 Enzyme Inhibitors

15.4 Ribozyme （Nonprotein Enzymes）

Part IV Industrial Fermentation

Chapter 16 Principles of Fermentation

16.1 Fermentation Principles

16.2 Fermentation Products and Application

16.3 Benefits from the Fermentation Industry

Chapter 17 Industrial Microorganisms

17.1 Types and Characters of Industrial Microorganisms

17.2 Breeding of Industrial Strains

17.3 Industrial Microbial Growth and Culture Media

17.4 Inoculation of Industrial Strains

17.5 Preservation of Industrial Strains

Chapter 18 Microbial Metabolism and Fermentation Kinetics

18.1 Metabolism Related toBasic Pathway

18.2 Metabolic Regulation and Control

18.3 Stoichiometry of Fermentation Process

18.4 Fermentation Kinetics

Chapter 19 Bioreactors and Auxiliary Equipments

19.1 Structure and Function of Bioreactors

19.2 Auxiliary Equipment

19.3 Probes and Sensors

Chapter 20 Fermentation DownStream Technology

20.1 General Aspects

20.2 Harvest and Pretreatment

20.3 Isolation and Purification

20.4 Product Polishing

20.5 Waste Treatment and Cleaner Production

Chapter 21 Progress in Fermentation Engineering

21.1 Approaches of Upstream Technology

21.2 Process Optima

21.3 Development of Downstream Technology

Appendix

Terms Translation

Related Nobel Laureates

Further Reading

后记