郭华荣主编的这本《细胞工程技术》主要内容分植物细胞工程和动物细胞工程两部分，既包含了植物细胞、组织和器官培养，动物细胞、组织和器官培养，动植物细胞融合，转基因动物和转基因植物，以及克隆动物等内容，还增加了细胞核重编程和诱导性胚胎干细胞这些新的概念和技术，以及细胞毒理、体外培养细胞的基因转染和表达等实用性很强的技术内容等。

PART Ⅰ CYTOTECHNOLOGY IN PLANTS

CHAPTER 1 INTRODUCTION FOR PLANT CELL, TISSUE AND ORGAN

CHAPTER 2 LABORATORY FACILITIES

CHAPTER 3 ASEPTIC TECHNIQUES

CHAPTER 4 MEDIA COMPOSITION AND PREPARATION

CHAPTER 5 INITIATION AND MAINTENANCE OF CALLUS

CHAPTER 60RGANOGENESIS

CHAPTER 7 CELL SUSPENSIONS

CHAPTER 8 SOMATIC EMBRYOGENESIS

CHAPTER 9 CULTURE OF ISOLATED ROOTS

CHAPTER 10 MICROPROPAGATION BY BUD PROLIFERATION

CHAPTER 11 ANTHER AND POLLEN CULTURES

CHAPTER 12 ISOLATION AND CULTURE OF PROTOPLASTS

CHAPTER 13 PROTOPLAST FUSION AND SOMATIC HYBRIDIZATION

CHAPTER I4 CRYOPRESERVATION OF GERMPLASM

CHAPTER 15 PRODUCTION OF SECONDARY METABOLITES

CHAPTER 16 TRANSGENIC PLANTS

PART Ⅱ CYTOTECHOLOGY IN ANIMALS

CHAPTER 17 INTRODUCTION TO ANIMAL CELL CULTURE

CHAPTER 18 BIOLOGY OF CULTURED CELLS

CHAPTER 19 EQUIPMENTS FOR ANIMAL CELL CULTURE

CHAPTER 20 MEDIA FOR ANIMAL

CHAPTER 21 PREPARATION AND STERILIZATION OF MEDIA

CHAPTER 22 SERUM-FREE MEDIA

CHAPTER 23 PRIMARY CELL CULTURE

CHAPTER 24 CELL LINES

CHAPTER 25 CELL CLONING AND SELECTION

CHAPTER 26 TRANSFORMATION OF CULTURED CELLS

CHAPTER 27 CHARACTERIZATION OF CELL LINES

CHAPTER 28 CONTAMINATION OF CULTURED CELLS

CHAPTER 29 CRYOPRESERVATION OF CUL TURED CELLS

CHAPTER 30 CYTOTOXOCITY

CHAPTER 31 EMBRYONIC STEM CELLS

CHAPTER 32 NUCLEAR REPROGRAMMING AND INDUCED PLURIPO-TENT STEM CELLS

CHAPTER 33 ORGAN CULTURE, HISTOTYPIC CULTURE AND ORGA-NOTYPIC CULTURE

CHAPTER 34 ANIMAL CELL FUSION

CHAPTER 35 ANIMAL CELL TRANSFECTION

CHAPTER 36 TRANSGENIC ANIMAL

CHAPTER 37 CLONED ANIMAL

GLOSSARY

REFERENCES