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书名 元基因组学--方法和步骤(影印版)/生命科学实验指南系列
分类 科学技术-自然科学-生物科学
作者 (德)斯特赖特W.R.//丹尼尔R.
出版社 科学出版社
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简介
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斯特赖特 W.R.和丹尼尔 R.所著的这本《元基因组学——方法和步骤(影印版)》为“生命科学实验指南系列”丛书之一,内容包括了元基因文库的构建,元基组文库的筛选技术和针对不同活性目标产物的筛选方法,可以帮助希望从事元基因组学研究的科技人员尽快建立相关的实验平台。

内容推荐

斯特赖特 W.R.和丹尼尔 R.所著的这本《元基因组学——方法和步骤(影印版)》涵盖了元基因组文库的构建、元基因组文库的筛选技术和针对不同活性目标产物的筛选方法,并且提供了详细实用的实验操作方法,可以帮助希望从事元基因组学研究的科技人员尽快建立相关的实验平台。

《元基因组学——方法和步骤(影印版)》适合于从事环境微生物学、微生物生态学等相关专业的高年级本科生、研究生,以及相关研究工作者等参考使用。

目录

Preface

Contributors

Molecular Methods to Study Complex Microbial Communities

Construction of Small-Insert and Large-Insert Metagenomic Libraries

Construction and Screening of Marine Metagenomic Libraries

Metagenomic Analysis of Isotopically Enriched DNA

Wide Host-Range Cloning for Functional Metagenomics

Cloning and Expression Vectors for a Gram-Positive Host, Streptomyces lividans

Heterologeus Gene Expression in the Hyperthermophilic Archaeon Sulfolobus solfataricus

Novel Tools for the Functional Expression of Metagenomic DNA

Screening of Functional Promoter from Metagenomic

DNA for Practical Use in Expression Systems

Substrate-Induced Gene Expression Screening: A Method

for High-Throughput Screening of Metagenome Libraries

Screens for Active and Stereoselective Hydrolytic Enzymes

Screening for Cellulase-Encoding Clones in Metagenomic Libraries

Screening Metagenomic Libraries for Laccase Activities

Screening for N-AHSL-Based-Signaling Interfering Enzymes

Identification of Molecular Markers to Follow Up the Bioremediation

of Sites Contaminated with Chlorinated Compounds

Methods for the Isolation of Genes Encoding Novel PHB

Cycle Enzymes from Complex Microbial Communities

Metagenomic Approaches to Identify and Isolate Bioactive

Natural Products from Microbiota of Marine Sponges

Screening for Novel Antibiotic Resistance Genes

Novel Metal Resistance Genes from Microorganisms:A Functional Metagenomic Approach

Retrieval of Full-Length Functional Genes Using Subtractive

Hybridization Magnetic Bead Capture

Detection and Isolation of Selected Genes of Interest from

Metagenomic Libraries by a DNA Microarray Approach

Application ofDNA Microarray for Screening Metagenome Library Clones

MetaGenomeThreader:. A Software Tool for Predicting

Genes in DNA-Sequences of Metagenome Projects

Index

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