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书名 病原生物学与免疫学实验(医学教育改革系列教材)(英文版)
分类 科学技术-医学-基础医学
作者 张力平
出版社 高等教育出版社
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张力平所著的《病原生物学与免疫学实验(医学教育改革系列教材)(英文版)》是医学教育改革系列教材,共分为两大模块,28个小单元,针对病原微生物,实验—医学—院校—免疫学—等内容进行全方位解析,是一本值得一看的医学用书。

目录

Part 1 Basic Experiments

Experiment 1 Observation of Bacterial Morphology

Ⅰ.Using Oil Immersion Lens of the Microscope

Ⅱ.Basic Morphology and Special Structurc of Bacteria

Experiment 2 Bacterial Staining and Microscopy

Ⅰ.Gram Stain

Ⅱ.Acid-fast Stain(Ziehl-Neelsen Method)

Experiment 3 Preparation of the Basic Medium

Experiment 4 Examination Methods of Parasitic Infections

Ⅰ.Direct Fecal Smear Method for Detecting Ascaris Eggs

Ⅱ.Examination for Ascaris I.arvae in Lung Tissue of Mice

Ⅲ.Saturated Brine Flotation Method for Detecting Hookworm Eggs

Ⅳ.Fecal Culture Method for Hookworm Larvac

Ⅴ.Cellophane Tape Method for Detecting Pinworm Eggs

Ⅵ.Direct Sputum Smear or Centrifugal Sedimentation Method for Detecting Paragonimus Eggs

Ⅶ.Squash Method for Detecting Striated Muscle Larvae of Trichmella

Ⅷ.Test of Concentration of Eggs by Sedimentation for Detecting S.Iaponicum Eggs

Ⅸ.The Miracidium Hatching Method for Detecting S.Japonicum

Ⅹ.Circumoval Precipitation Test(COPT) for Diagnosing Sc.histosomiasis

Ⅺ.Direct Stool Smear with Iodine-stain for Detecting Amoeba Cysts

Ⅻ.Blood Smear Method for Detecting Malarial Parasites

Experiment5 Agglutination Reaction

Ⅰ.Dircct Agglutination Reaction-Identification of the ABO Blood Group System

Ⅱ.Indirect Agglutination Reaction——-Anti-streptolysin O Test

Ⅲ.Indirect Agglutination Inhibition Reaction Latex Pregnancy Test

Experiment 6 Precipitation Reaction

Ⅰ.Single Radial Immunodiffusion Test

Ⅱ.Doublelmmunodiffusion Test

Experiment 7 Enzyme-Iinked Immunosorbent Assay(Sandwich ELISA Assay)

Part 2 Comprehensive Experiments

 Unit 1 Isolation of Microorganisms from the Environment and Human Body

Experiment 8 Bacteria in the Air and Ultraviolet Radiation

Experiment 9 Bacteria on the Finger Tip and Solid Surface

 Unit 2 Detection of Skin Infections

Experiment 10 Cultural Characteristics of Bacteria and Fungi(Teaching Demonstration)

Ⅰ.Clostridium Perfringens in the Milk Medium.

Ⅱ.Fungiin Sabouraud Dextrose Medium

Experiment 11 Observation of Plasmodium,Toxoplasma Gondii,Trichinella Spiralis,Echinococcus Granulosus and Insecta

Ⅰ.Plasmodium

Ⅱ.Toxoplasma Gondii

Ⅲ.Trichinella Spiralis

Ⅳ.Echinococcus Granulosus

Ⅴ.Insecta

 Unit 3 Detection of Respiratory Infections

Experiment 12 Isolation & Identification of Pyogenic Cocci

Ⅰ.Isolation and Culture of Mimic Pus Swab and Throat Swab

Ⅱ.Coagulase Test

Experiment 13 Cultural Characteristics of Respiratory Infected Bacteria

Ⅰ.The Colonies of Pathogenic(Jocci on Blood Agar Plates(Teaching Demonstration)

Ⅱ.Mycobacterium Tuberculosis in Lowenstein Jensen Solid Medium(Teaching Demonstration)

Experiment 14 Observation of Paragonimus Westermani

 Unit 4 Detection of Enteric Infections

Experiment 15 Isolation and Identification of Pathogenic Enterobacteria

Ⅰ.Isolation of Artificial Fecal Specimens

Ⅱ.Biochemical Activities of Enteric Pathogens-Kligler Iron Agar Test

Ⅲ.Serologic Identification of Enteric Pathogens

Experiment 16 Widal Test

Experiment 17 Observation of Ascaris lumbricoides,Hookworms,Enterobius Vermicularis,Taenia,Entamoeba and Cr yptos poridium

Ⅰ.Ascaris Lumbricoides

Ⅱ.Hookworms(Ancylostoma Duodenale and Necator Americanus)

Ⅲ.Enterobius Vermicularis

Ⅳ.Intestinal Cestodes(Taenia Solium and Taenia Saginata)

Ⅴ.Intestinal Protozoa(Entamoeba Histolytica and Entamoeba Coli)

Ⅵ.Cryptosporidium

 Unit 5 Detection of Hepatic Infections

Experiment 18 Observation of Clonorchis Sinensis

Experiment 19 Observation of Schistosoma Ja ponicum

 Unit 6 Detection of Genitourinary Tract Infections

Experiment 20 Observation of Chlamydia Trachomatis Inclusion

 Unit 7 AntimicrobiaIResistance

Experiment 21 Bacterial Conjugation:the Transfer of Plasmids Coding for Antibiotic Resistance

Experiment 22 Antibiotic Sensitivity Test

 Unit 8 Immune Cells Function Assay

Experiment 23 Macrophage Phagocytosis Assay

Experiment 24 Neutrophil Phagocytosis Assay

Experiment 25 Plaque Forming Cells Assay (PFC Assay)

Experiment 26 Lymphocyte Transformation Assay

Experiment 27 Classification of T Cell Subset and FACS Analysis

Experiment 28 Cytokine Measurement by ELISPOT

Appendix

 Appendix 1 Commonly Used Dye and Staining

 Appendix 2 Media

 Appendix 3 Commonly Used Reagents and Buffers

Index

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