本书包括20个再生药物及传递常用实验项目，首先介绍了再生医学的实验场地要求，其次介绍了组织工程构建时种子细胞的分离和培养，重点介绍原代细胞分离、纯化和培养的方法，之后重点介绍了几种常用的药物传递系统的构建，包括纳米粒、脂质体、微囊等，同时介绍了几种常用的组织工程支架构建方法，此外，结合最新3D打印技术，介绍同轴打印的方法构建组织工程化血管，最后介绍了几种常见再生药物及传递的仪器使用方法。本书的出版能帮助留学生更好地进行再生医学和药物、基因输送相关实验，对提高留学生教学质量及来华留学生课程建设具有积极意义。

Chapter 1 Establishment of Tissue Engineering Laboratory
Laboratory design for cell culture
Rules and regulations for cell culture room
Chapter 2 Seed Cell Culture and Identification
Basic principles of in vitro cell culture
Experiment 1 Isolation and culture assay of primary cells
Experiment 2 Primary cell subculture and its passage assay
Experiment 3 Freezing and resuscitation of cells
Experiment 4 MTT method to measure cell proliferation rate
Experiment 5 Immunostaining assay
Experiment 6 DAPI nuclear fluorescent labeling assay
Experiment 7 Cell migration assay
Experiment 8 SDS-polyacrylamide gel electrophoresis assay
Experiment 9 Isolation and cultivation of rat bone marrow mesenchymal stem cells
Chapter 3 Fabrication of Three-dimensional Scaffolds
Experiment 10 Solvent casting assay
Experiment 11 Gel pressing method assay
Experiment 12 Preparation of injectable thermosensitive hydrogel
Experiment 13 Freeze-drying method assay
Experiment 14 Three-dimensional tissue printing technique assay
Experiment 15 Coaxial bioprinting of dual network bioink to prepare blood vessels
Chapter 4 Growth Factor Delivery Systems for Regenerative Medicine
Experiment 16 Preparation of hydrogel for growth factor delivery
Experiment 17 Application of hydrophobic polymers in GF transport
Experiment 18 Growth factor perfusion delivery assay
Experiment 19 pH sensitive drug delivery assay
Experiment 20 Magnetically responsive drug delivery assay
Chapter 5 Use of Common Instruments and Equipment for Tissue
Engineering
Workbench
Inverted fluorescence microscope
Carbon dioxide (CO2) incubator
Bioreactor
Laser confocal microscopy
References